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Quantifiable viremia was lower in ZIKV infections compared with CHIKV or DENV (mean 4.70 vs 6.42 and 5.84 log ZIKV, CHIKV, and DENV result in similar clinical presentations, and coinfections may be relatively common.

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Our findings illustrate the need for accurate, multiplex diagnostics for patient care and epidemiologic surveillance.

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Zika virus (ZIKV), chikungunya virus (CHIKV), and dengue virus (DENV) cocirculate in Nicaragua.

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Acute-phase serum samples from 346 patients with a suspected arboviral illness were tested using a multiplex real-time reverse-transcription polymerase chain reaction for ZIKV, CHIKV, and DENV.


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